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rat/mouse epo quantikine elisa kit  (R&D Systems)


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    R&D Systems rat/mouse epo quantikine elisa kit
    Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal <t>EPO</t> and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by <t>ELISA.</t> (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.
    Rat/Mouse Epo Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantikine+rat+epo+elisa+kit/elisa+kit/pmc07849576-50-7-12
    Average 90 stars, based on 1 article reviews
    rat/mouse epo quantikine elisa kit - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "C-FGF23 peptide alleviates hypoferremia during acute inflammation"

    Article Title: C-FGF23 peptide alleviates hypoferremia during acute inflammation

    Journal: Haematologica

    doi: 10.3324/haematol.2019.237040

    Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal EPO and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by ELISA. (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.
    Figure Legend Snippet: Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal EPO and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by ELISA. (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.

    Techniques Used: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Transformation Assay, Comparison

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Isocitrate ameliorates anemia by suppressing the erythroid iron restriction response
    Article Snippet: Serum iron analysis was performed as described ( 17 ) using the Ferene Serum Iron/UIBC kit (Thermo Scientific). .. Serum erythropoietin and IFN-γ were measured using the Quantikine Rat EPO ELISA kit and Quantikine Rat IFN-γ ELISA kit (R&D Systems). ..

    Article Title: Isocitrate ameliorates anemia by suppressing the erythroid iron restriction response
    Article Snippet: Serum iron analysis was performed as described (17) using the Ferene Serum Iron/UIBC kit (Thermo Scientific). .. Serum erythropoietin and IFN-γ were measured using the Quantikine Rat EPO ELISA kit and Quantikine Rat IFN-γ ELISA kit (R&D Systems). ..

    Article Title: Dynamics of Erythropoietic Markers in Response to Hypoxia in Rats
    Article Snippet: .. Plasma concentration of endogenous EPO was measured using Quantikine rat EPO ELISA kit (R&D Systems, Minneapolis, MN, USA). ..

    Clinical Proteomics:

    Article Title: Dynamics of Erythropoietic Markers in Response to Hypoxia in Rats
    Article Snippet: .. Plasma concentration of endogenous EPO was measured using Quantikine rat EPO ELISA kit (R&D Systems, Minneapolis, MN, USA). ..

    Concentration Assay:

    Article Title: Dynamics of Erythropoietic Markers in Response to Hypoxia in Rats
    Article Snippet: .. Plasma concentration of endogenous EPO was measured using Quantikine rat EPO ELISA kit (R&D Systems, Minneapolis, MN, USA). ..



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    Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal <t>EPO</t> and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by <t>ELISA.</t> (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.
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    Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal <t>EPO</t> and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by <t>ELISA.</t> (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.
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    Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal EPO and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by ELISA. (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.

    Journal: Haematologica

    Article Title: C-FGF23 peptide alleviates hypoferremia during acute inflammation

    doi: 10.3324/haematol.2019.237040

    Figure Lengend Snippet: Inhibition of fibroblast growth factor 23 (FGF23) signaling increases renal and extra-renal EPO and EpoR mRNA expression under lipopolysaccharide (LPS)-induced hypoferremia. C57BL/6J wild-type mice were treated with C-tail FGF23 (1 mg/kg, indicated as FGF23 BL) or vehicle (HEPES buffer) for 8 hours (h). Mice were then challenged with LPS (intraperitoneal 50 mg/kg) or vehicle (0.9% NaCl) for 4 h. (A-C) Quantitative real-time polymerase chain reaction (qRT-PCR) for renal expression of (A) Epo , (B) EpoR , and (C) Hif2 α. (D) Serum concentration of Epo measured by ELISA. (E-H) qRT-PCR for Epo expression in (E) spleen and (F) liver, and EpoR expression in (G) spleen and (H) liver. Data are expressed as fold change (2-DDCt) relative to housekeeping genes Gapdh or Hprt . Samples were measured in duplicates (n=5-7 per group). Data are represented as mean+standard deviation. All data were analyzed for normality by Shapiro-Wilk test and equivalence of variance using Levene’s test. When the samples did not show normal distribution, they were aligned in RANK transformation, and confirmed for normality. As the samples showed normal distribution, two-way ANOVA was performed with Bonferroni’s multiple comparison test (B, F, and H). The samples not in normal distribution were analyzed with non-parametric Kruskal-Wallis test (A, C-E, and G). Ctl: control (vehicle), ns: not significant, * P <0.05, ** P <0.01, *** P <0.001.

    Article Snippet: Serum EPO levels were measured using the Rat/Mouse EPO Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA).

    Techniques: Inhibition, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation, Transformation Assay, Comparison